Studying proteins and their interactions in concentrated and crowded environments via tracer sedimentation equilibrium and static light scattering
The general theories of sedimentation equilibrium (SE) and static light scattering (SLS) have been recently extended to be applicable to mixtures of interacting solutes at arbitrary concentration. We have developed experimental procedures for the acquisition of SE and SLS data in concentrated solutions suitable for analysis, and provided examples of the use of these measurements to characterize protein […]
Hybrid gelation processes in enzymatically gelled biopolymers: rheology, nanostructure and cellular response
Hydrogels obtained from the chemical or physical association of macromolecules are an important area of material science, due to the wide array of design possibilities and countless biomedical applications, for instance as skin substitutes, adhesives, or drug delivery matrices. Through a careful choice of the macromolecules, the molecular associations that maintain the hydrogel 3D network can be selected […]
Nanosight
http://www.youtube.com/watch?v=DMdvmHI4wsg
Protein-protein interactions, viscosity and injectability in multi protein co-formulations comprising Bevacizumab and recombinant HSA
It is well-known that recombinant HSA (rHSA) has the ability to stabilize proteins in in solution preventing protein adsorption, protein aggregation and protein oxidation. At low protein drug concentrations (< 1mg/mL) the mechanism of action is relatively well understood and the beneficial effect is expected to act through coating of hydrophobic and hydrophilic surfaces on the primary packaging […]
Protein-protein interactions in monoclonal antibody formulations: Effect on solution viscosity
A large number of monoclonal antibodies under pharmaceutical development are low potency molecules and require several mg/kg body weight doses. For patient convenience and in-home administration to reduce cost, self-injections via subcutaneous route are preferred. The Subcutaneous route of administration poses a volume restriction (< 1.5 mL), which necessitates the use of formulations containing high concentration of the drug. […]
Crowded Solutions of Globular Proteins and Monoclonal Antibodies: pH, Composition and Excipient Effects on Inter-molecular Interactions and Solution Shear Rheology
Solutions of globular proteins and monoclonal antibodies (mAbs) possess complex pH and concentration dependence of viscosity (h) and diffusivity (D). Despite these complexities, dilute solution properties like diffusion interaction parameter (kD) and second virial coefficient (B22) have recently been proposed to predict colloidal stability and at high protein concentrations. In this two-part talk, I will first discuss recent studies […]
Dr. Leonardo Borras
Alcon Labs (Novartis company) – leonardo.borras@alconlabs.com The Importance of Early Biophysical Characterization of Antibodies in the Lead Selection Process A critical aspect of the antibody lead selection process is assessing and understanding the biophysical properties of antibody lead candidates. An early characterization helps to reduce or eliminate development issues while allowing for ideal lead selection. We have […]
Prof. Mike Williamson
Dept of Molecular Biology and Biotechnology, University of Sheffield – m.williamson@sheffield.ac.uk Taming protein interactions All proteins have evolved to interact, and in most cases this interaction is with other proteins. Sometimes the interactions are strong, but more often they are weak (Kd > 10 μM), especially in the crowded environment of the cell. Such interactions are […]
Professor Michele Vendruscolo
Cambridge University – mv245@cam.ac.uk Sequence-based prediction of protein solubility As increasing evidence indicates that the sequences of proteins encode not just their folding but also their solubility and aggregation [1,2], there is great interest in identifying the amino acid code responsible for the maintenance of solubility. We have been developing computational methods to predict different aspects of […]
Avacta Analytical
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